Gram staining is a common method used to differentiate between two groups of bacteria depending on their different cell wall structure. To remember it .. mnemonics
LONG PRESENTATION
LONG is related to gm -ve and PPT related to gm +ve
LONG PPT
What is the difference then in the cell wall structure ?
Gram +ve VS gram -ve
Gram +ve | Gram –ve |
Stain blue or purple | Stain red or rose pink |
Techoic acid | Lipopolysaccharide (LPS) in outer membrane |
Peptidoglycan layer is thick | Peptidoglycan layer is thin |
Exotoxin is a metabolic product | Endotoxin is a metabolic product |
Exotoxin is thermolabile (heat sensitive) destroyed at a temperature over 60 oC | Endotoxin is more toxic than exotoxin, destroyed at higher temperatures. |
Exotoxin is a high molecular weight protein. | Endotoxin a complex structure made up of phospholipid, polysaccharides and protein. |